1998-1999 Undergraduate internship.

My main internship was performed at Molecular Genetics, Rijksuniversiteit Groningen, under supervision of Dr. Kees Leenhouts. The goal of the project was to investigate the binding properties of several cell wall and cell membrane binding domains fused to heterologous proteins and to display these proteins at the bacterial cell surface, in such a way that the bacterial cells could be used as an oral vaccine. My work resulted in two publications (see below) and the system developed is now actively used by Mucosis, a clinical stage Dutch biotechnology company developing innovative mucosal vaccines for oral delivery.

  1. Steen A*, Buist G*, Leenhouts KJ, El Khattabi M, Grijpstra F, Zomer AL, Venema G, Kuipers OP, Kok J.Cell wall attachment of a widely distributed peptidoglycan binding domain is hindered by cell wall constituents.J Biol Chem. 2003 Jun 27;278(26):23874-81. Epub 2003 Apr 8.
  2. Ramasamy R*, Yasawardena S, Zomer A, Venema G, Kok J, Leenhouts K. Immunogenicity of a malaria parasite antigen displayed by Lactococcus lactis in oral immunisations. Vaccine. 2006 May 1;24(18):3900-8. Epub 2006 Mar 2.


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