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Discovering novel bile protection systems in Bifidobacterium breve UCC2003 through functional genomics.

Appl Environ Microbiol. 2012 Feb;78(4):1123-31. Epub 2011 Dec 9.

Discovering novel bile protection systems in Bifidobacterium breve UCC2003 through functional genomics.

Ruiz L*, Zomer A, O'Connell-Motherway M, van Sinderen D, Margolles A.

Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias-CSIC, Villaviciosa, Asturias, Spain.

Tolerance of gut commensals to bile salt exposure is an important feature for their survival in and colonization of the intestinal environment. A transcriptomic approach was employed to study the response of Bifidobacterium breve UCC2003 to bile, allowing the identification of a number of bile-induced genes with a range of predicted functions. The potential roles of a selection of these bile-inducible genes in bile protection were analyzed following heterologous expression in Lactococcus lactis. Genes encoding three transport systems belonging to the major facilitator superfamily (MFS), Bbr_0838, Bbr_0832, and Bbr_1756, and three ABC-type transporters, Bbr_0406-0407, Bbr_1804-1805, and Bbr_1826-1827, were thus investigated and shown to provide enhanced resistance and survival to bile exposure. This work significantly improves our understanding as to how bifidobacteria respond to and survive bile exposure.

PMID:22156415

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